<body>
<form id="form1" runat="server">
<table>
<tr>
<td style="text-align:right">UserName:</td>
<td>
<asp:TextBox runat="server" ID="UserName"></asp:TextBox>
<asp:RequiredFieldValidator ID="RequiredFieldValidator1" runat="server" ControlToValidate="UserName" ErrorMessage="Enter UserName" ForeColor="Red" SetFocusOnError="True"></asp:RequiredFieldValidator>
</td>
</tr>
<tr>
<td style="text-align:right">Email:</td>
<td><asp:TextBox runat="server" ID="Email"></asp:TextBox>
<asp:RequiredFieldValidator ID="RequiredFieldValidator2" runat="server" ControlToValidate="Email" ErrorMessage="Enter Email Address" ForeColor="Red"></asp:RequiredFieldValidator>
<asp:RegularExpressionValidator ID="RegularExpressionValidator1" runat="server" ControlToValidate="Email" ErrorMessage="Enter Correct Email Address" ForeColor="Red" SetFocusOnError="True" ValidationExpression="\w+([-+.']\w+)*@\w+([-.]\w+)*\.\w+([-.]\w+)*"></asp:RegularExpressionValidator>
</td>
</tr>
<tr>
<td style="text-align:right">Gender</td>
<td>
<asp:RadioButtonList runat="server">
<asp:ListItem>Male</asp:ListItem>
<asp:ListItem>Female</asp:ListItem>
</asp:RadioButtonList>
</td>
</tr>
</table>
</form>
</body>